Job description

Détail de l'offre

Informations générales

Entité de rattachement

Le Commissariat à l'énergie atomique et aux énergies alternatives (CEA) est un organisme public de recherche.

Acteur majeur de la recherche, du développement et de l'innovation, le CEA intervient dans le cadre de ses quatre missions :
. la défense et la sécurité
. l'énergie nucléaire (fission et fusion)
. la recherche technologique pour l'industrie
. la recherche fondamentale (sciences de la matière et sciences de la vie).

Avec ses 16000 salariés -techniciens, ingénieurs, chercheurs, et personnel en soutien à la recherche- le CEA participe à de nombreux projets de collaboration aux côtés de ses partenaires académiques et industriels.

Référence

2019-11213

Description du poste

Domaine

Biologie, biophysique et biochimie

Contrat

Stage

Intitulé de l'offre

INTERNSHIP - Nanodelivery systems for gene therapy H/F

Sujet de stage

A position is available in Cell Biology/Nanomedicine for a M2 student within the LSMB laboratory at the CEA LETI health department.
The internship project would be focused on nanomedicine. We are looking for a motivated candidate to help the pre-clinical development of a novel non-viral system for gene therapy. The project relies on a lipid nanocarrier technology to transport a nucleic acid (in that case a small interfering RNA) into target cells, enabling the specific knock-down of genes involved in the inflammatory response.

Durée du contrat (en mois)

Description de l'offre

In Europe alone, more than 3 million people live with inflammatory bowel diseases (IBD), which currently have no cure. Patient care relies mostly on handling the symptoms using intravenously injected monoclonal antibody to alleviate the patient’s immune response. Although patients are responding to these treatments, the improvement is only temporary in many cases whereas numerous adverse effects are degrading their quality of life.

The NEW DEAL project is proposing an alternative to current treatments for IBD to improve patient care. The ultimate goal of the project is to develop a drug delivery system using lipid nanoparticles to deliver a small interfering RNA (siRNA) targeting the genes involved in the inflammatory response (JAK1/JAK3) and relieve patient from acute local inflammation. This novel approach, combined with a specific formulation for oral delivery, would allow for the release of the siRNA-containing nanotherapeutic vector at the right intestinal compartment.

Within the NEW DEAL H2020 project, the candidate will participate to thein vitrotesting of new lipid nanoparticle formulations for the delivery of the JAK1/JAK3 targeting siRNA. The candidate will perform experiments to evaluate and characterise the activity of siRNA/lipid nanoparticles complexes in mammalian cells. Absorption and internalisation of the nanocomplexes will be assessed and quantified in relevant cell models by automated confocal imaging (High-Content Screening). A single-cell analysis might have to be developed by the candidate, using machine learning, to analyze the image dataset recorded. In addition, the candidate will be responsible to assay the functional activity of the nanocomplexes on target gene expression, using standard established techniques (western blotting).

More details are available on the NEW DEAL H2020 project at: https://newdeal-project.eu/

Moyens / Méthodes / Logiciels

Tissue culture, electrophoresis & western-blot, confocal microscopy, image analysis

Desired profile

Profil du candidat

The candidate must be a Master 2 student with a major in Biochemistry, Biology or Biotechnology.
This position would require a previous experience with biochemical analytic methods (Western-blot) and cell biology (mammalian tissue culture). Familiarity with imaging techniques (confocal microscopy), image analysis (Image J, Cell Profiler) or programming (Python) would be a plus. The candidate should have good experimental rigor, good communication skills and affinity for teamwork.

Offers “CEA”